genomic dna isolation blood system Search Results


beyomag blood genomic dna isolation kit  (Beyotime)
99
Beyotime beyomag blood genomic dna isolation kit
Beyomag Blood Genomic Dna Isolation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/BeyoMag+Blood+Genomic+DNA+Isolation+Kit+with+Magnetic+Beads/pm41250997-408-15-24
Average 99 stars, based on 1 article reviews
beyomag blood genomic dna isolation kit - by Bioz Stars, 2026-11
99/100 stars
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genomic dna human blood  (Promega)
90
Promega genomic dna human blood
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genomic Dna Human Blood, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/wizard+genomic+blood+dna+isolation+kit/pmc02262070-202-0-5
Average 90 stars, based on 1 article reviews
genomic dna human blood - by Bioz Stars, 2026-11
90/100 stars
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prep™ blood cell culture dna isolation kit  (BioNano Genomics)
90
BioNano Genomics prep™ blood cell culture dna isolation kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Prep™ Blood Cell Culture Dna Isolation Kit, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/sp+blood+++cell+culture+dna+isolation+kit/pmc10378017-49-10-9
Average 90 stars, based on 1 article reviews
prep™ blood cell culture dna isolation kit - by Bioz Stars, 2026-11
90/100 stars
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dna isolation protocol bionano prep sp frozen human blood  (BioNano Genomics)
90
BioNano Genomics dna isolation protocol bionano prep sp frozen human blood
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Dna Isolation Protocol Bionano Prep Sp Frozen Human Blood, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/sp+frozen+human+blood+dna+isolation+protocol/pmc09184658-69-17-18
Average 90 stars, based on 1 article reviews
dna isolation protocol bionano prep sp frozen human blood - by Bioz Stars, 2026-11
90/100 stars
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dna isolation kit bionano-prep-sp-g2-bma/pb  (BioNano Genomics)
90
BioNano Genomics dna isolation kit bionano-prep-sp-g2-bma/pb
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Dna Isolation Kit Bionano Prep Sp G2 Bma/Pb, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/dna+isolation+kit+bionano+prep+sp+blood+and+cell+culture/pmc12123785-49-18-18
Average 90 stars, based on 1 article reviews
dna isolation kit bionano-prep-sp-g2-bma/pb - by Bioz Stars, 2026-11
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sp blood and cell culture dna isolation kit bionano pn 80042  (BioNano Genomics)
90
BioNano Genomics sp blood and cell culture dna isolation kit bionano pn 80042
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Sp Blood And Cell Culture Dna Isolation Kit Bionano Pn 80042, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/sp+blood+and+cell+culture+dna+isolation+kit+bionano+pn+80042/pm38326333-75-28-28
Average 90 stars, based on 1 article reviews
sp blood and cell culture dna isolation kit bionano pn 80042 - by Bioz Stars, 2026-11
90/100 stars
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total dna isolation kit magcore genomic dna whole blood kit for genotyping  (RBC Bioscience)
90
RBC Bioscience total dna isolation kit magcore genomic dna whole blood kit for genotyping
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Total Dna Isolation Kit Magcore Genomic Dna Whole Blood Kit For Genotyping, supplied by RBC Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/total+dna+isolation+kit+magcore+genomic+dna+whole+blood+kit+for+genotyping/pmc11202224-104-14-25
Average 90 stars, based on 1 article reviews
total dna isolation kit magcore genomic dna whole blood kit for genotyping - by Bioz Stars, 2026-11
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sp blood and cell culture dna isolation kit v2  (BioNano Genomics)
90
BioNano Genomics sp blood and cell culture dna isolation kit v2
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Sp Blood And Cell Culture Dna Isolation Kit V2, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/sp+blood+++cell+culture+dna+isolation+kit+v2/pmc11477233-192-11-11
Average 90 stars, based on 1 article reviews
sp blood and cell culture dna isolation kit v2 - by Bioz Stars, 2026-11
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genomic dna isolated from peripheral blood  (Integragen sa)
90
Integragen sa genomic dna isolated from peripheral blood
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genomic Dna Isolated From Peripheral Blood, supplied by Integragen sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
genomic dna isolated from peripheral blood - by Bioz Stars, 2026-11
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purebind blood genomic dna isolation kit  (Ocean NanoTech)
90
Ocean NanoTech purebind blood genomic dna isolation kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Purebind Blood Genomic Dna Isolation Kit, supplied by Ocean NanoTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/purebind+blood+genomic+dna+isolation+kit/pm38521763-446-6-12
Average 90 stars, based on 1 article reviews
purebind blood genomic dna isolation kit - by Bioz Stars, 2026-11
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blood and cell culture dna isolation kit 80004  (BioNano Genomics)
90
BioNano Genomics blood and cell culture dna isolation kit 80004
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Blood And Cell Culture Dna Isolation Kit 80004, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+isolation+blood+system/blood+and+cell+culture+dna+isolation+kit+80004/pmc10152236-56-15-15
Average 90 stars, based on 1 article reviews
blood and cell culture dna isolation kit 80004 - by Bioz Stars, 2026-11
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dna isolation kit for mammalian blood wizard genomic dna purification kit  (Promega)
90
Promega dna isolation kit for mammalian blood wizard genomic dna purification kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Dna Isolation Kit For Mammalian Blood Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna isolation kit for mammalian blood wizard genomic dna purification kit - by Bioz Stars, 2026-11
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Image Search Results


Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Journal: BMC Genomics

Article Title: Human glycolipid transfer protein ( GLTP ) genes: organization, transcriptional status and evolution

doi: 10.1186/1471-2164-9-72

Figure Lengend Snippet: Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Article Snippet: Genomic DNA from human blood (Promega) or isolated from various cell lines (Sigma-Aldrich, GenElute™ mammalian genomic DNA miniprep kit) was bisulfite converted using manufacturer's recommendations (CpGenome Fast; Chemicon, Temecula, CA).

Techniques: Methylation, Clone Assay, Reverse Transcription Polymerase Chain Reaction, Combined Bisulfite Restriction Analysis Assay, Agarose Gel Electrophoresis, Amplification, Sequencing, Negative Control, Molecular Weight